A plasmid (vector) designed for use in bacterial cells is different from a plasmid (vector) designed for use in yeast cells. Listed below are three DNA features found on both plasmid types. If the DNA sequence of each feature could be the same on the two different plasmids, answer yes. If the DNA sequence would be different on the two plasmids, answer no.

Cloning site

Yes
No
Origin of replication

Yes
No
Selectable marker

Yes
No

Cloning site: Yes

The cloning site refers to a specific DNA sequence where the foreign DNA of interest can be inserted into the plasmid. This sequence is usually the same or compatible in both bacterial and yeast plasmids, allowing for the insertion of DNA in a similar manner.

Origin of replication: No
The origin of replication (ORI) is a DNA sequence necessary for the initiation of DNA replication within the host cell. Bacterial and yeast cells have different mechanisms for DNA replication, so the ORI sequences on the plasmids designed for bacterial and yeast cells would be different.

Selectable marker: Yes
A selectable marker is a genetic element that allows for selection of cells containing the plasmid. Examples of selectable markers include antibiotic resistance genes or auxotrophic markers. While the specific DNA sequence of the selectable markers may differ between bacterial and yeast plasmids, the concept and functionality of the selectable marker is the same, allowing cells with the plasmid to be identified and selected for.