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April 20, 2014

Homework Help: biology

Posted by Anonymous on Thursday, May 2, 2013 at 2:49am.

A common first step in the making of a DNA library is to obtain DNA from many of your chosen organisms and cut this source DNA with a restriction enzyme. It is likely that some genes will contain sites for the chosen restriction enzyme, and in this case, your DNA library will not have a complete set of genes from your chosen source. There are several ways to avoid this problem, but one solution is to add smaller amounts of the restriction enzyme or decrease the time that the DNA is exposed to active enzyme. In the box below, explain why this approach may allow you to construct a more complete library.

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