This is the homework exercise:

You want to add 0.13 ul of DNA to 4.87 ul of extraction mix to have a final total of 5.0 ul of sample. But you don't want to make 0.13 aliquots because it is too small. Make a dilution to accomodate for the small aliquot.

Any help would be great thanks

To accommodate the small aliquot of 0.13 ul, you can make a dilution by adding a larger volume of a diluent (e.g., water or buffer) to the DNA sample. Here's how you can calculate the dilution:

1. First, determine the dilution factor you need. Since you want to add 0.13 ul of DNA to a total volume of 5.0 ul, the dilution factor can be calculated as:

Dilution factor = Desired final volume / Volume of DNA to be added
Dilution factor = 5.0 ul / 0.13 ul

2. Now, determine the volume of diluent to be added. Multiply the dilution factor by the volume of DNA to be added:

Volume of diluent = Dilution factor x Volume of DNA
Volume of diluent = (5.0 ul / 0.13 ul) x 0.13 ul

3. Finally, subtract the volume of DNA from the total desired volume to find the volume of diluent required:

Volume of diluent = Desired final volume - Volume of DNA
Volume of diluent = 5.0 ul - 0.13 ul

By following these steps, you can dilute the 0.13 ul DNA aliquot to a larger volume that is more easily pipettable while still maintaining the desired DNA concentration.