Thursday
April 17, 2014

Homework Help: chem biochem bio

Posted by mel on Sunday, March 10, 2013 at 5:45pm.

You have the following stock solutions and solid chemicals in your lab:

1.5M NaCL
0.2M Tris buffer, pH 8.0
10 mg/ml Bovine serum albumin (BSA)
MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure)

How would you make 250 ml of the following solution???

0.2M NaCl
0.05M Tris, pH 8.0
0.25 mg/ml BSA
25mM MgCl2

If i understand the NaCL can be determined by c1v1=c2v2 but the BSA MgCL2 and Tris I dont understand, plus is the total suppossed to add to be 250 ml?

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