Wednesday
July 23, 2014

Homework Help: Dilution

Posted by John on Friday, February 15, 2013 at 12:43pm.

Please, if possible, check if my method of dilution is correct
Problem: Given a very concentrated liquid PBS, dilute it to 1:15,000

My approach is to use serial dilution
First: dilute it 15x (50ul of PBS + 750ul of water)... this will give 1:15

Second: dilute 10xfold more (100ul PBS from step 1 + 900ul water)...this will give 1:150

Third: dilute anotehr 10x fold more (100ul PBS from step 2 + 900ul water)...this will give 1:1500

Fourth: dilute anotehr 10x fold more (100ul PBS from step 3 + 900ul water)...this will give 1:15000

Is my approach and calculation correct?

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