You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer.
I know this so far::
1- Glucose
DF = C2 / C1 = 5% (DIVIDE) 20% = 0.25
V1 - DF X V2 = 0.25 X 300 ml = 75 ml
2 - Tris Stock = 1 M = 1000 mM
DF = C2 / C1 = 100 mM (DIVIDE) 1000 mM = 0.1
V1 - DF X V2 = 0.1 X 300 ml = 30 ml
Is this right so far?
Yes, both are right. Good work.
I am doing the same problem.
I got as far as you, including:
DF = C2/C1 = 10 mM /500mM = 0.02 EDTA
V1= DF x V2 = 0.02 x 300mL = 6 mL
...Now i am not sure what to do with this information. I would appreciate the help.
add up the three volumes:
75mL + 30mL + 6 mL = 111mL
The question tells us that the total final volume of the buffer is 300mL, so add enough water to your solute to bring it up to that desired volume:
300mL - 111mL = 189mL
So you would combine 111mL of the solutes to 189mL of the diluent, which is water, to get a final volume of 300mL.
Yes, your calculations for glucose and tris are correct so far.
Now let's move on to the calculation for EDTA:
3 - EDTA Stock = 0.5 M = 500 mM
DF = C2 / C1 = 10 mM (DIVIDE) 500 mM = 0.02
V1 - DF X V2 = 0.02 X 300 ml = 6 ml
So, you will need to add 75 ml of the 20% glucose stock solution, 30 ml of the 1M tris stock solution, and 6 ml of the 0.5M EDTA stock solution to make the desired buffer. Finally, you can fill up the remaining volume with distilled water to reach the final volume of 300 ml.