Tuesday
July 22, 2014

Homework Help: Chemistry 3A

Posted by Katie on Friday, February 1, 2013 at 4:58pm.

You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer.

I know this so far::

1- Glucose
DF = C2 / C1 = 5% (DIVIDE) 20% = 0.25

V1 - DF X V2 = 0.25 X 300 ml = 75 ml


2 - Tris Stock = 1 M = 1000 mM

DF = C2 / C1 = 100 mM (DIVIDE) 1000 mM = 0.1

V1 - DF X V2 = 0.1 X 300 ml = 30 ml

Is this right so far?

Answer this Question

First Name:
School Subject:
Answer:

Related Questions

Science - You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-...
chemistry - first task is to prepare 250 ml of 3 M glucose. Then You are ...
Chemistry - The molecular mass of glucose is 180g/mol. you prepare a stock ...
chem - A student placed 12.0grams of glucose in a flask added enough water to ...
chemistry - How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required ...
Chemistry - Which of the solutions has the greatest osmotic pressure? Choose one...
chemistry - Prepare 150 ml of a reaction buffer containing 0.75% MgCl2, 35mM ...
Biochem - Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) ...
Chemistry - A student placed 14.0 of glucose () in a volumetric flask, added ...
chemistry - A student placed 11.5 of glucose () in a volumetric flask, added ...

Search
Members