Monday
March 30, 2015

Homework Help: Chemistry 3A

Posted by Katie on Friday, February 1, 2013 at 4:58pm.

You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer.

I know this so far::

1- Glucose
DF = C2 / C1 = 5% (DIVIDE) 20% = 0.25

V1 - DF X V2 = 0.25 X 300 ml = 75 ml


2 - Tris Stock = 1 M = 1000 mM

DF = C2 / C1 = 100 mM (DIVIDE) 1000 mM = 0.1

V1 - DF X V2 = 0.1 X 300 ml = 30 ml

Is this right so far?

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