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April 20, 2014

Homework Help: Biology

Posted by Emily on Monday, January 28, 2013 at 1:10pm.

In a lab experiment, we experimented with 1% glucose solution and Sorenson's solution to determine pH levels and whatnot; the other part of the lab was to determine which one, if either, was a buffering solution. According to the notes my instructor gave us, a buffers are mixtures of chemicals (a weak acid and its conjugate base) that prevent a change in the number of available hydrogen ions or hydroxyl ions in solution (in other words, it helps maintain the pH of the solution.)

So we first measured the pH of the 1% glucose solution after every 10 mL of HCl added until there were 50 mL of HCl added total. We then did the same thing, but instead of glucose we used Sorenson's solution, and added HCl to that until 50 mL total were added. The thing that confused me though was that the pH level of each solution rose at somewhat the same increments as each other. Should this have happened? Because I don't see how one could determine which one was a buffering solution just based off of these numbers because they both rose at almost the same rate.

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