Suppose you are a scientist trying to help people who cannot produce an enzyme needed for proper digestion. How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Did this dude just say to google it?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
Darn, guess I shoulda just googled it ._.
If they are on here maybe they already tried to google it?
somebody please help me :'(
To create transformed bacteria that produce the enzyme needed for proper digestion, you can utilize genetic engineering techniques. Here's how you could approach it:
1. Identify the gene: First, you need to identify the gene responsible for producing the enzyme required for digestion. This can be done through research, studying the human genome, or analyzing similar enzymes found in other organisms.
2. Clone the gene: Once you have identified the gene, you can clone it by isolating the specific DNA sequence that encodes the enzyme using techniques like Polymerase Chain Reaction (PCR). This process will give you multiple copies of the desired gene.
3. Create a recombinant DNA: Next, you need to insert the cloned gene into a vector, which is a vehicle for transferring genes. Common vectors used for bacterial transformation include plasmids, circular DNA molecules capable of independent replication within bacterial cells.
4. Insert the recombinant DNA into bacteria: Take the recombinant DNA and introduce it into the bacteria of choice. This process is known as transformation. One common method for bacterial transformation is the heat shock method, where the bacteria are briefly exposed to a high temperature followed by rapid cooling.
5. Select transformed bacteria: After transformation, you need to select only the bacteria that have taken up the recombinant DNA containing the gene of interest. This selection is achieved by incorporating a selectable marker, such as an antibiotic resistance gene, into the vector along with the gene of interest. The presence of the marker allows for the identification and growth of only the transformed bacteria.
6. Verify gene expression: Confirm that the transformed bacteria are producing the desired enzyme. This can be done through various tests, such as enzyme activity assays or protein expression analysis, to ensure the enzyme is being properly synthesized by the transformed bacteria.
7. Optimize production: Finally, you can optimize the conditions for gene expression and enzyme production in the transformed bacteria. Factors like temperature, pH, and growth medium composition can be adjusted to enhance enzyme production, resulting in a higher yield of the enzyme needed for digestion.
By following these steps, you can use genetic engineering techniques to create transformed bacteria that produce the enzyme required for proper digestion.
Since this is not my area of expertise, I searched Google under the key words "genetic transformation of enzyme producing bacteria" to get these possible sources:
http://www.google.com/search?client=safari&rls=en&q=genetic+transformation+of+enzyme+producing+bacteria&ie=UTF-8&oe=UTF-8
In the future, you can find the information you desire more quickly, if you use appropriate key words to do your own search. Also see http://hanlib.sou.edu/searchtools/.