for template DNA (non-GMO,GMO,and test food) we set up a PCR reaction that included "plant' primers. Assume that all three foods had usable plant DNA. Which template DNA (non-GMO,GMO, test food) would you expect to show successful amplification of the "plant" target sequence? I said yes to all three foods would amplification because of the DNA amplifies the chloroplast DNA sequence.
Based on the information provided, it is expected that all three template DNAs (non-GMO, GMO, and test food) would show successful amplification of the "plant" target sequence if they contain usable plant DNA. This is because the PCR reaction includes "plant" primers, which are designed to specifically amplify the target plant DNA sequence. Therefore, if the template DNAs from all three sources contain plant DNA, they would be expected to amplify the chloroplast DNA sequence.
To determine which template DNA (non-GMO, GMO, or test food) would show successful amplification of the "plant" target sequence, you need to consider the presence of chloroplast DNA in each food sample.
Chloroplasts are organelles found in plants that contain their own DNA. Amplification of the "plant" target sequence would be possible if the food samples contain intact chloroplast DNA.
In this case, you mentioned that all three foods had usable plant DNA. Assuming that the plant DNA in each sample includes intact chloroplast DNA, it is reasonable to expect successful amplification of the "plant" target sequence for all three DNA samples (non-GMO, GMO, and test food) because they should all contain chloroplast DNA.
However, it is important to note that this assumption may not always hold true, especially for GMO foods. Genetic modification can involve various techniques, including nuclear genome manipulation, which may affect the presence or stability of chloroplast DNA. Therefore, additional confirmation or analysis specific to GMOs may be required to accurately determine successful amplification in GMO samples.