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March 27, 2017

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You wish to perform a restriction digest in a 20uL volume. Your DNA is 0.20 ug/uL and you wish to digest 1.0 ug. Your restriction enzyme is provided as 0.2 U/uL. Fill in the appropriate volumes: H2O, 10Xbuffer, DNA, and restriction enzyme.

Please show work. I'm more interested in how to get the answer. Thanks.

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