determine the volume of 45mM ethanol needed to make 1700 microliters that has a final ethanol concentration of 26.5mM?
Use c1v1 = c2v2
1700 x 26.5 = 45 x uL
Solve for uL.
To determine the volume of 45mM ethanol needed to make 1700 microliters with a final concentration of 26.5mM, we can use the concept of dilution.
The formula for dilution is:
C1V1 = C2V2
Where:
C1 = initial concentration of the solute (before dilution)
V1 = initial volume of the solute (before dilution)
C2 = final concentration of the solute (after dilution)
V2 = final volume of the solute (after dilution)
Let's plug in the values given in the question:
C1 = 45mM
V1 = unknown (we need to find this)
C2 = 26.5mM
V2 = 1700 microliters
Now we can rearrange the formula to solve for V1:
V1 = (C2 * V2) / C1
V1 = (26.5mM * 1700 microliters) / 45mM
Before we proceed, note that the units of mM and microliters need to be converted to a consistent unit. Let's convert everything to milliliters (mL):
V1 = (26.5mM * 1.7 mL) / 45mM
Simplifying further:
V1 = 995 mL / 45
V1 ≈ 22.11 mL
Therefore, approximately 22.11 mL of 45mM ethanol is needed to make 1700 microliters with a final ethanol concentration of 26.5mM.