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Posted by on Wednesday, September 26, 2012 at 1:05am.

Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5.

Useful information:
pKa Tris = 8.1
Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1
Formula weight of Tris hydrochloride (acid form) = 158.0

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