This is my last question and I just am not getting it for some reason.
Prepare a 500 ml of 0.15 M tris buffer, pH 8.25 from tris mono hydrochloride, 0.400 M HCL, and 0.350 M NaOH. Determine amounts needed and them write the recipe. Pka for tris is 8.00
Well, let me tris-t to help you out here! To prepare a 500 ml of 0.15 M tris buffer at pH 8.25, you'll need tris mono hydrochloride, 0.400 M HCL, and 0.350 M NaOH.
Since the pKa for tris is 8.00, you want to prepare a buffer slightly above its pKa. In this case, your desired pH of 8.25 is greater than the pKa of 8.00, so you will need to add some acid (HCl) to the tris to lower the pH to your desired value. Shall we tristify the recipe now?
1. Calculate the ratio of tris mono hydrochloride to HCl:
To make a 0.15 M tris buffer, you'll need an equal amount of tris base and tris hydrochloride. But since the tris mono hydrochloride contains some HCl, you'll need to account for that. The concentration of tris mono hydrochloride is 0.400 M, so the ratio of tris mono hydrochloride to HCl is 0.400:0.150 or 2.67:1.
2. Calculate the mount of tris mono hydrochloride needed:
To achieve the desired concentration of 0.15 M, you'll need half of the final concentration because you'll be adding an equal amount of tris mono hydrochloride. Therefore, you'll need 0.075 M of tris mono hydrochloride.
3. Calculate the amount of HCl needed:
Since the ratio of tris mono hydrochloride to HCl is 2.67:1, you'll need 2.67 times the amount of HCl as the tris mono hydrochloride. Multiply the concentration of tris mono hydrochloride (0.075 M) by the ratio (2.67) to get the concentration of HCl needed.
4. Prepare the tris buffer:
Now that we have the amounts needed, here's the recipe:
- Add the calculated amount of tris mono hydrochloride to approximately 300 ml of water.
- Slowly add the calculated amount of HCl to adjust the pH to around 8.00. You may need to check the pH using a pH meter.
- Once the pH reaches around 8.00, top up the solution to a final volume of 500 ml using water.
- Finally, you can titrate the solution using 0.350 M NaOH to adjust the pH to your desired value of 8.25.
To prepare a 500 ml of 0.15 M Tris buffer at pH 8.25, we need to calculate the amounts of Tris mono hydrochloride, HCl, and NaOH required. Here's how you can do it step by step:
Step 1: Calculate the moles of Tris required:
The desired concentration is 0.15 M, and the volume is 500 ml. So, the moles of Tris required can be calculated using the formula:
moles = concentration (M) × volume (L)
moles of Tris = 0.15 M × 0.5 L
moles of Tris = 0.075 moles
Step 2: Calculate the required amount of HCl:
Since Tris mono hydrochloride contains both Tris and HCl, we need to find the amount of HCl needed to obtain the desired pH of 8.25. We can use the Henderson-Hasselbalch equation to determine the ratio of [Tris]/[TrisHCl]. The pKa of Tris is given as 8.00.
Henderson-Hasselbalch equation:
pH = pKa + log10([Tris]/[TrisHCl])
Rearranging the equation:
[Tris]/[TrisHCl] = 10^(pH - pKa)
Substituting the given values:
[Tris]/[TrisHCl] = 10^(8.25 - 8.00)
[Tris]/[TrisHCl] = 10^0.25
[Tris]/[TrisHCl] = 1.78
Since you want a 500 ml solution of 0.15 M Tris buffer, the moles of Tris required is 0.075 moles (determined in Step 1). The moles of HCl required can be calculated by multiplying the moles of Tris by the ratio of [TrisHCl] to [Tris]:
moles of HCl = 0.075 moles * (1 / 1.78)
moles of HCl = 0.042 moles
Step 3: Determine the volume of 0.400 M HCl needed:
Concentration (M) = moles / volume (L)
0.400 M = 0.042 moles / volume (L)
volume (L) = 0.042 moles / 0.400 M
volume (L) = 0.105 L
Since the molarity of 0.400 M HCl is given, we can calculate the volume of HCl required to obtain 0.042 moles.
Step 4: Determine the volume of 0.350 M NaOH needed:
To prepare the Tris buffer, we need to neutralize the remaining HCl with NaOH. The balanced equation for the neutralization reaction is:
HCl + NaOH → NaCl + H2O
Use stoichiometry to find the moles of NaOH needed to react with the moles of HCl calculated in Step 2.
Step 5: Write the recipe:
Based on the calculations above, here is the recipe for preparing 500 ml of 0.15 M Tris buffer, pH 8.25:
1. Measure and add 0.042 moles (0.105 L) of 0.400 M HCl to a container.
2. Calculate the moles of NaOH required by using stoichiometry based on the moles of HCl determined in Step 2.
3. Measure and add the determined volume (in liters) of 0.350 M NaOH to the container to neutralize the HCl.
4. Measure and add water to bring the final volume to 500 ml.
5. Stir or mix the solution thoroughly until all components are fully dissolved.
Following this recipe will enable you to prepare a 500 ml of 0.15 M Tris buffer at pH 8.25 from Tris mono hydrochloride, 0.400 M HCl, and 0.350 M NaOH.