posted by Hannah on .
In TLC, why do you spot multiple times? Select one or more correct answers.
A. To fill the capillary almost instantly by capillary action.
B. So the developing chamber solvent does not evaporate.
C. The higher compound concentration facilitates visualization.
D. To acheive a high concentration of sample in a small spot.
THERE IS MORE THAN ONE ANSWER CORRECT HERE. I KNOW IT IS D, BUT THERE IS ONE MORE CORRECT ANSWER. WHAT IS IT? THANKS!
I'm not an expert on TLC but the way I see it it can't be A or B. Capillaries are not involved and multiple spotting has nothing to do (at least I don't think so) with the solvent evaporation. I didn't pick C earlier because that applies only to "VISIBLE" components and TLC is good for separating components, too, that are not visible (for example amino acids). However, if there is an answer in addition to D it must be C.
thanks :] it is correct. D and C are the answers