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Posted by on Thursday, July 5, 2012 at 9:33am.

I have 3 mg/mL AMP in 50ul stock solution; How do I dilute it to 50ug in 50ul

thanks you

  • chemistry - , Thursday, July 5, 2012 at 1:06pm

    Isn't 3 mg/mL = 3000 ug/1000 uL? Then 50 uL contains
    3000 ug x (50 uL/1000 uL) = 150 ug/50 uL = 3 ug/uL.
    If you want 50 ug/50 uL, that means you want 50 ug from the stock soln? How many uL does it take to obtain 50 ug?
    3 ug/uL x ?uL = 50 ug
    ?uL = 50/3 = 16.67 uL and dilute to 50 uL.
    Check my work.

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