Give the sequence of a reverse primer used to insert a BamHI site (GGATCC) at the 3' end of the following sequence:
5' CTGAACGTCCAAGGCTTAA 3'
3' GACTTGCAGGTTCCGAATT 5'
Make sure you indicate your primers polarity.
To insert a BamHI site (GGATCC) at the 3' end of a given sequence, we need to design a reverse primer. A reverse primer is oriented in the 3' to 5' direction of the target DNA sequence.
Given sequence: 5' CTGAACGTCCAAGGCTTAA 3'
Reverse complement: 3' GACTTGCAGGTTCCGAATT 5'
The next step is to determine the reverse primer sequence. We know that BamHI recognition site is GGATCC, which is a reverse complement to itself. Therefore, our reverse primer needs to be the exact reverse complement of GGATCC.
Reverse complement of GGATCC: GGATCC
Therefore, the reverse primer sequence, with the polarity indicated, is:
5' GGATCC 3'
This primer should be used to insert a BamHI site (GGATCC) at the 3' end of the given sequence.