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April 25, 2015

Homework Help: Biochemistry

Posted by Peter on Sunday, January 29, 2012 at 6:19pm.

1. MES (2-(N-Morpholino)ethanesulfonic acid) is a common buffer used in biochemistry labs to stabilize proteins. The pKa for MES is 6.09.
a. What is the pH of 1 liter of a 200 mM MES aqueous solution? Clue: you will need to solve a quadratic equation. (2.5 points)
b. How much solid NaOH (in grams) needs to be added to the MES solution to make a buffer at pH 6.5? (1.5 points)

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