Posted by umesh on .
how to prepare 1M,50ml buffer solution by taking Na2HPO4 and NaH2PO4.
In biochemistry the molarity of the buffer is the sum of both the acid and the conjugate base. Your post just gives the total but not the breakdown; therefore, I would suggest 50 mL(0.05L) for 0.5M each. That is 0.5 x 0.05 = 0.025 moles.
g each = moles x molar mass.