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Chemistry-Bob please help

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Posted by anne on Friday, October 14, 2011 at 6:30pm.


Hi, I have a question on HPLC (high performance liquid chromatography).
The procedure says to use a
4.6 x 150mm x 3um column.
The relative retention time of Impurity B with reference to the main peak is about 0.5 (i.e the ratio of the retention times is 0.5)
If I use a 4.6 x 100mm x 2.7um column, will the relative retention time of Impurity B with reference to the main peak still be about 0.5?

  • Chemistry-Bob please help -

    My calculations below, show yes, about the same, but it has been a long time since I did that. I have asked DrBob to take a look at it to, I am uncertain if he has done any HPLC work.

  • Chemistry-Bob please help -

    http://www.jiskha.com/display.cgi?id=1318631421

  • Chemistry-Bob please help -

    I haven't done any HPLC work.

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