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July 22, 2014

Homework Help: Chemistry-Bob please help

Posted by anne on Friday, October 14, 2011 at 10:19pm.

Posted by anne on Friday, October 14, 2011 at 6:30pm.


Hi, I have a question on HPLC (high performance liquid chromatography).
The procedure says to use a
4.6 x 150mm x 3um column.
The relative retention time of Impurity B with reference to the main peak is about 0.5 (i.e the ratio of the retention times is 0.5)
If I use a 4.6 x 100mm x 2.7um column, will the relative retention time of Impurity B with reference to the main peak still be about 0.5?

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