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Supercoiling of DNA can occur (choose all that apply):

A. when double stranded DNA is overwound
B. when double stranded DNA is underwound
C. when double stranded DNA is heated above it's Tm
D. when DNA is treated with nucleases

and

Generally, cellular DNA is:
A. Slightly underwound
B. Slightly overwound
C. Chemically unstable in basic solutions
D.Single stranded

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