How to prepare 1000 nM stock solution using Tam (1 gm) Mol Weight=563.6
To prepare a 1000 nM (nanomolar) stock solution using Tam (Tamoxifen) with a molecular weight of 563.6 g/mol, you need to follow these steps:
1. Calculate the molar mass of Tam (Tamoxifen): Given that the molecular weight of Tamoxifen is 563.6 g/mol, this is the mass of 1 mole of Tamoxifen.
2. Calculate the required mass of Tamoxifen: To prepare a 1000 nM stock solution, you need to determine the amount of Tamoxifen needed. Since the desired concentration is in nanomoles per liter (nM), you will require 1000 nanomoles of Tamoxifen per liter of solution.
1000 nM = 1000 x 10^(-9) moles per liter = 1 x 10^(-6) moles per liter
Therefore, you need 1 x 10^(-6) moles (or 1 micro-mole) of Tamoxifen per liter of solution.
To convert from moles to grams, multiply the moles by the molar mass of Tamoxifen:
Mass of Tamoxifen = 1 x 10^(-6) moles x 563.6 g/mol
3. Calculate the required mass of Tamoxifen:
Mass of Tamoxifen = 1 x 10^(-6) moles x 563.6 g/mol
= 0.0005636 g = 0.5636 mg
Therefore, you need 0.5636 mg of Tamoxifen per liter of solution to obtain a 1000 nM stock solution.
It's important to note that when preparing a stock solution, you should consider the solvent to be used and its volume for the dilution. Additionally, make sure to accurately measure the mass of Tamoxifen and dissolve it thoroughly in the desired solvent before adjusting the final volume to reach 1 liter using additional solvent. Following good laboratory practices and referring to specific protocols or guidelines is advisable when preparing stock solutions.