Can we use an AR grade chemical for UV spectrophotometric analysis?

Or is it a must to use a chemical that is specially designated (SpectrAR grade) for UV analysis?

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In UV spectrophotometric analysis, it is generally recommended to use chemicals that are specially designated for this purpose, such as SpectrAR grade chemicals. These chemicals are specifically manufactured and tested to ensure high purity and low impurities, which are crucial for accurate and reliable UV analysis.

However, if SpectrAR grade chemicals are not available or if you have a limited budget, you may consider using AR (Analytical Reagent) grade chemicals as an alternative. AR grade chemicals are also of high purity, but they may contain slightly higher impurities compared to SpectrAR grade chemicals. Hence, using AR grade chemicals might introduce some level of uncertainty or variability in the UV analysis results.

To decide whether AR grade chemicals are suitable for your specific UV spectrophotometric analysis, several factors need to be considered:

1. Nature of the analysis: If the UV analysis requires high precision or demands accurate measurements, it is highly recommended to use SpectrAR grade chemicals to minimize any potential interference from impurities.

2. Sensitivity of the analysis: If the UV analysis is highly sensitive or requires detection of low concentrations, using SpectrAR grade chemicals is advisable to reduce any background noise or spurious signals that might arise from impurities in AR grade chemicals.

3. Validation requirements: If your analysis needs to be validated or compliant with specific regulations or industry standards, it is generally preferred to use SpectrAR grade chemicals, as they are specifically manufactured to meet those standards.

In summary, while it is generally recommended to use SpectrAR grade chemicals for UV spectrophotometric analysis, the decision to use AR grade chemicals depends on factors such as the nature of the analysis, sensitivity requirements, and validation standards. It is important to carefully assess these factors and consider the potential impact of impurities before making a decision.