March 28, 2017

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Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for Tris (hydroxymethyl) aminomethane, pKa = 8.1; for acetic acid, pKa = 4.76)

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