Posted by Rose on Wednesday, January 12, 2011 at 8:05pm.
Don't I need the concn of the buffer?
The TRIS-HCl buffer is 0.1 M.
This is done the same way the phosphate buffer is done. The only difference is the pKa value for TRIS. If you have a follow up question, please repost at the top of the page. I have trouble finding these when they get so far at the bottom of the list.
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