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Posted by on Wednesday, January 12, 2011 at 8:05pm.

If I take 5mL of a TRIS/HCL buffer with a pH of 7.4 and add 1mL of 1M HCL, how do I determine the new pH?

  • Biochemisty - , Wednesday, January 12, 2011 at 11:57pm

    Don't I need the concn of the buffer?

  • Biochemisty - , Thursday, January 13, 2011 at 12:08am

    The TRIS-HCl buffer is 0.1 M.

  • Biochemisty - , Thursday, January 13, 2011 at 5:24pm

    This is done the same way the phosphate buffer is done. The only difference is the pKa value for TRIS. If you have a follow up question, please repost at the top of the page. I have trouble finding these when they get so far at the bottom of the list.

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