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April 24, 2014

Homework Help: biochemistry

Posted by ameera on Saturday, January 8, 2011 at 11:46am.

The hydrolysis of one mole of sulphate ester produces one mole of protons.In a reaction tube containing 1.0ml of 0.02M TRIS buffer,pH8.08,sulphate ester was incubated with sulphatase for 10 min.At the end of reaction,the pH was reduced to 7.97.Calculated the amount of sulphate ester that has been hydrolyzed in 10 min.give your answer in micromoles of sulphate ester.

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