Posted by ameera on Saturday, January 8, 2011 at 11:46am.
I would do something like this. Check my thinking. If the solution is 1 mL x 0.02 M TRIS buffer and the pH is 8.08, that means equal moles TRIS and TRIS*HCl (substitute into the HH equation and base/acid = 1 if you don't see this right away). 1 mL x 0.02 M = 0.02 mmoles buffer or 0.01 mmoles TRIS and 0.01 mmoles TRIS*HCl
...............TRIS + H^+ ==> TRIS*HCl
initial mmoles..0.01...0....0.01 mmole
change...........-x...+x.......+x
final..........0.01-x...+x......0.01+x
Substitute into the HH equation and solve for x.
7.97 = 8.08 + log [(0.01-x)/(0.01+x)]
x will be in mmoles H^+ which will be the same as mmoles of the ester hydrolyzed. You will need to change to micromoles ester hydrolyzed.
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