chemistry - chromatography
posted by dan on .
Hello, can you tell me why different colors have different rf values
It isn't the color, itself. It's the chemical makeup that produces the color of the sample.
We used three different color dyes in both alcohol and water. So its the chemical makeup of each color that determines the Rf value?
You don't say what kind of chromatography this is.
Yes, it's the formula for each dye as well as the kind of column (if GC), the kind of stationary phase (if TLC), etc. That is, if the chemical of the dye binds to the adsorbent of the stationary phase, then the dye will move very slowly and not very far. If however, there is little attraction between the stationary phase and the sample, it will move faster and farther.