Quick question: which methods can be used to get DNA into cells? you can choose more than 1 answer
A: Apply a high voltage
B: Shoot DNA into the cells
C: Infect with viruses
D: Inject with a needle
E: All of the above
F: None of the above
Is it all of the above? If so can anyone provide reasoning why? thx
The correct answer is E: All of the above.
To understand why all of these methods are valid for introducing DNA into cells, let's examine each one:
A: Apply a high voltage (Electroporation) - This method involves subjecting cells to a brief, high-voltage electrical pulse. The electrical field disrupts the cell membrane, creating temporary pores through which DNA can enter the cells.
B: Shoot DNA into the cells (Biolistics or Particle Bombardment) - This method utilizes small particles (such as gold or tungsten) coated with DNA. These particles are accelerated and directly shot into the cells. Upon entry, the DNA is released and taken up by the cells.
C: Infect with viruses (Viral Transduction) - Certain viruses, such as retroviruses and adenoviruses, can be modified to carry specific DNA sequences. By infecting the cells with these modified viruses, the viral DNA is delivered into the cells, allowing for its integration into the host genome.
D: Inject with a needle (Microinjection) - This method involves using a fine needle to directly inject DNA into the nucleus or cytoplasm of cells. It requires precise handling and is often used for single-cell manipulation or in vitro fertilization.
It is essential to note that all of these methods can be used to deliver DNA into cells, but their suitability may depend on factors such as the cell type, the purpose of the experiment, and the specific requirements of the DNA being delivered.