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Microbiolgy- Please Help!

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You wish to study a variant [a close relative] of the Tobacco Mosaic virus. Specifically you want to characterize the capsid and genetic component as well as determine the viral load required to cause a disease state.

a. What methodologies might you use to isolate and characterize this microbe?

b. How will you determine the LD50 of this viral particle?

c. Describe the mechanism of infection for a typical enveloped virus.

I have been searching on the internet, but I am not having much luck. I think I'm ok with part c, but I can't find anything on a or b, besides that the LD50 is the dose required to kill half the members of a population. I would greatly appreciate any help.

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