February 25, 2017

Homework Help: Biochemistry

Posted by Angel on Tuesday, September 14, 2010 at 10:48pm.


HEPES, is a commonly used component of physiological buffers. The PKa of the protonated amino group of HEPES is 7.55 at 25C.

Describe the preparation of 1.00L of 0.100M HEPES buffer, pH 8.00, from the sodium salt of the basic form of HEPES (MW 260.3), 6.0M HCl and deionized water

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