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Posted by on Sunday, March 21, 2010 at 9:55pm.

ISOLATION OF CHLOROPHYLL AND CAROTENOID PIGMENTS FROM SPINACH:
What would happen to the Rf values of the pigments if you were to increase the relative concentration of acetone in the developing solvent ?

I said the Rf values would be higher because Acetone is more polar than hexane ... so the components would move faster across the TLC plate. We used 70%hexane-30% acetone for preparing our development chamber. ... I would like to know if my answer is right and if not what's the right answer please. Thanks

  • Organic Chemistry Lab - , Sunday, March 21, 2010 at 10:02pm

    I'm no TLC man but my thinking is the same as yours.

  • Organic Chemistry Lab - , Sunday, June 10, 2012 at 4:53pm

    I'm pretty sure pigments travel further when they are less polar. Since acetone is more polar then the rf values would be less.

  • Organic Chemistry Lab - , Wednesday, October 23, 2013 at 1:47am

    Thanks

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