ISOLATION OF CHLOROPHYLL AND CAROTENOID PIGMENTS FROM SPINACH:
What would happen to the Rf values of the pigments if you were to increase the relative concentration of acetone in the developing solvent ?
I said the Rf values would be higher because Acetone is more polar than hexane ... so the components would move faster across the TLC plate. We used 70%hexane-30% acetone for preparing our development chamber. ... I would like to know if my answer is right and if not what's the right answer please. Thanks
Organic Chemistry Lab - DrBob222, Sunday, March 21, 2010 at 10:02pm
I'm no TLC man but my thinking is the same as yours.
Organic Chemistry Lab - hmmm, Sunday, June 10, 2012 at 4:53pm
I'm pretty sure pigments travel further when they are less polar. Since acetone is more polar then the rf values would be less.
Organic Chemistry Lab - Kieu, Wednesday, October 23, 2013 at 1:47am