with amino acid ionisation staes do the r groups/side chains deprotonate first or the nh3+ group im talking about aspartic acid if that's relevant

Thank you

Without capitalization and periods and other appropriate punctuation, your question is gibberish. I may not be able to answer it anyway, but as it stands, I don't know what it says.

To determine the order of deprotonation for aspartic acid, it's crucial to understand the pKa values of the relevant functional groups. The lower the pKa value, the more acidic the functional group is, and it will tend to donate its proton first when the pH is increased.

In aspartic acid, there are three relevant functional groups:

1. Carboxylic Acid (COOH): The pKa value for carboxylic acids is typically around 2-3. In aspartic acid, the carboxylic acid group donates its proton first as the pH increases.

2. Amino Group (NH2): The pKa value for amino groups is usually around 9-10. Therefore, the amino group will donate its proton at a higher pH compared to the carboxylic acid group.

3. Side Chain (R Group): The side chain of aspartic acid contains a carboxyl group (-COOH). The pKa value for the side-chain carboxyl group can vary depending on its microenvironment. Generally, it falls within the range of 3-5. Consequently, the side chain carboxyl group donates its proton after the carboxylic acid group.

Thus, the order of deprotonation for aspartic acid with increasing pH is:

1. Deprotonation of the carboxylic acid group (-COOH) to form the carboxylate anion (-COO-).
2. Deprotonation of the side chain carboxyl group to form the carboxylate anion in the side chain (-R-COO-).
3. Deprotonation of the amino group (NH3+) to form the amino group (-NH2).

It's important to note that at physiological pH (~7.4), both the carboxylic acid group and the side chain carboxyl group are deprotonated, while the amino group retains its protonated form (+NH3+).