February 19, 2017

Homework Help: Bioanalytical Chemistry

Posted by Michael on Tuesday, October 27, 2009 at 12:37am.

Two proteins are separated by capillary electrophoresis. The two proteins have the following physical characteristics:
Protein A: net charge = +6; MW = 64,500; partial specific volume = 0.700 cm3 / g
Protein B: net charge = +3; MW = 35,000; partial specific volume = 0.700 cm3 / g
The separation is carried out in a capillary that is 100 cm long, and 30,000 V is used for the separation. Which protein will reach the end of the capillary first, and how long will it take to migrate the length of the capillary? Assume that there is no electroosmotic flow. Show your work. (e = 1.6x10-19 C; viscosity of water = 0.0114g/cm-s; J = N-m = kg m2 /sec2; V = J/C)

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