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April 25, 2015

Homework Help: Bioanalytical Chemistry

Posted by Michael on Tuesday, October 27, 2009 at 12:32am.

Enzyme X is immobilized to a support and is used to separate substrate Y from a mixture of other compounds. In a separate experiment the rate constant for the dissociation of substrate Y from enzyme X was determined to be 1.3 x 10-5 s-1. Suggest mobile phase conditions that would be best for eluting substrate Y after washing away the interfering compounds.

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