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August 2, 2015

Homework Help: Biology

Posted by Anonymous on Monday, October 12, 2009 at 2:10pm.

The absorbance of a protein solution measured in a 1.0 cm cuvette at 278 nm was 1.145. The protein content of that solution, calculated from quantitative amino acid analysis, was 256 micrograms/mL. Calculate the extinction coefficient of the protein in unitsbof mL/mg/cm. Assume that the molecular mass of the protein was 13,000. Calculate the molar extinction coefficient.

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