Wednesday
April 23, 2014

Homework Help: biology

Posted by Jenn on Saturday, July 18, 2009 at 5:24pm.

Which bacterial plates control for:
1) If the E. coli cells were transferred correctly:
2) If the E. coli cells can grow on LB:
3) If the Ampicillin is working correctly:
4) If the “stock” E. coli cells were contaminated with plasmid:

Possible choices for each number are:
"-"LB
"+"LB
"-"LB/Amp
"+"LB/Amp
"+"LB/Amp/Sugar

(LB is a growth medium)
("+" means the plate contains pGLO gene, which gives the bacteria resistance to Amp; "-" means it does not have pGLO)
(Amp is ampicillin)
(sugar gives fluorescent glow in presence of pGLO)

Please help me out. Thank you!

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