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Assuming pKa values of 3.8 for the a-COOH and 8.5 for the a-amino groups and that the numbers in parentheses indicate the pKa values of side chain groups, at what pH would electrophoresis provide the best resolution of the following dipeptides?

His (6.0) – Ser

Gly – Lys (10.0)

I think I'm missing the theory behind this. If the side chain and the pH are both acidic, does that mean it's better?

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