Posted by Jack on .
You wish to produce a human enzyme, protein A, by introducing its gene into bacteria.The genetically engineered bacteria make large amounts of protein A, but it is in the
form of an insoluble aggregate with no enzymatic activity. Which of the following procedures might help you to obtain soluble, enzymatically active protein? (Hint: Some
proteins require molecular chaperones in order to fold properly within the environment of the
(a) Treat the insoluble aggregate with a protease.
(b) Make the bacteria overproduce chaperone proteins in addition to protein A.
(c) Heat the protein aggregate to denature all proteins, then cool the mixture.
(d) None of the above.
I'm leaning towards (a) as my answer, but I'm not too sure.
Hopefully you can use something here: