February 22, 2017

Homework Help: Biology

Posted by Christiaan on Tuesday, November 11, 2008 at 5:42am.

For one of our lab sessions, we had to isolate DNA from E. Coli bacteria. During one of the steps in the isolation process, we had to use SDS(Sodium lauryl sulfate) at 60°C. One of the questions was:

Why do we use SDS, and why at this temperature.

I already found out that SDS is a detergent, and is therefor helping to release intracellular components.
But why is it being used at 60°C?

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