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July 29, 2015

Homework Help: Science - Chromatography

Posted by Veronica on Sunday, October 5, 2008 at 11:06pm.

I performed a TLC of plant pigments in my lab class with a solvent of petroleum ether/ acetone (7:3) and used a silica gel plate.
It is expected that the most polar pigments (chlorophyll) will be located near the bottom and the carotenoids near top.
In my experiment, it was the exact opposite - the green bands (belonging to chlorophyll) were located on the top and the yellow bands (belonging to the carotenoids) were on the bottom.
Can any one explain where I could have gone wrong in my experiment? Is a possibility that there was water in the solvent?

Any help would be greatly appreciated! Thanks!

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