The following chemicals have the indicated formula weight Surcose = 342.3g; Potassium dihydrogen=136.09; and Tris=121.14

Also the following solution already made: 20%SDS, 5M NaCl, 500mM EDTA. You have a 40% glycerol solution, and 10X TBE. List the step to make the following buffers:
a. 100ml of a 10% glycerol solution
b. 40ml 0.5% agarose in 1X TBE solution.

a. To make 100ml of a 10% glycerol solution, follow these steps:

Step 1: Calculate the amount of glycerol needed.
- From the given information, the 40% glycerol solution is available.
- We need to find out how much of this solution to take in order to obtain 10g of glycerol.

Calculation:
- Let's assume x ml of the 40% glycerol solution is needed.
- The density of the 40% glycerol solution is not given, so we will assume it is approximately 1g/ml.
- Therefore, 40% glycerol solution contains 40g of glycerol in 100ml.
- We need to find x in the following equation: (40g/100ml) × x = 10g
- Solving for x, we get:
x = (10g × 100ml) / 40g
x = 25ml

So, we need 25ml of the 40% glycerol solution to make 100ml of a 10% glycerol solution.

Step 2: Add glycerol to a container.
- Take a container or beaker that can hold at least 100ml of liquid.

Step 3: Measure out the required amount of the 40% glycerol solution.
- Pour 25ml of the 40% glycerol solution into the container.

Step 4: Add distilled water.
- Add distilled water to the container until the total volume reaches 100ml.
- Ensure you mix the solution well to ensure uniform distribution of glycerol throughout.

b. To make 40ml of a 0.5% agarose in 1X TBE solution, follow these steps:

Step 1: Calculate the amount of agarose needed.
- From the given information, the concentration of the agarose solution required is 0.5%.
- We need to find out how much agarose powder to take.

Calculation:
- 0.5% agarose means 0.5g agarose in 100ml of solution.
- We need to find out how much agarose is needed for 40ml of solution.

- Let's assume y g of agarose is needed for 40ml of solution.
- Using the above calculation, we can set up the equation:
(0.5g/100ml) × 40ml = y g
0.2g = y g

So, we will need 0.2g of agarose powder to make 40ml of a 0.5% agarose solution.

Step 2: Measure out the required amount of agarose powder.
- Accurately weigh 0.2g of agarose powder using a balance.

Step 3: Add agarose to a container.
- Take a container or beaker that can hold at least 40ml of liquid.

Step 4: Add 1X TBE buffer.
- Pour 40ml of the 10X TBE solution into the container.

Step 5: Dissolve the agarose.
- Heat the container containing the agarose and TBE buffer in a microwave or a water bath until the agarose is completely dissolved.
(Take caution, as agarose solutions can become hot.)

Step 6: Allow the solution to cool and solidify.
- Once the agarose is completely dissolved, allow the solution to cool to a temperature where it is still liquid but can be poured into casting trays.
- Pour the liquid agarose solution into casting trays and insert the comb if necessary.
- Let the solution solidify completely before using it for gel electrophoresis.

To make the requested buffers, you will need to perform some calculations to determine the amount of each chemical to add. Here are the steps to make the buffers:

a. 100ml of a 10% glycerol solution:

1. Determine the amount of glycerol needed:
- Desired final volume: 100ml
- Desired glycerol concentration: 10%
- Formula weight of glycerol: Approximately 92.09g/mol

To calculate the amount of glycerol needed, use the formula:
Amount (in grams) = (Desired concentration / 100) x Desired volume x Formula weight

Plugging in the values, we get:
Amount (in grams) = (10 / 100) x 100 x 92.09

2. Calculate the volume of the 40% glycerol solution needed:
- Concentration of 40% glycerol solution: 40%
- Volume of 40% glycerol solution = Amount (in grams) / (Concentration / 100)

Plugging in the values, we get:
Volume of 40% glycerol solution = Amount (in grams) / (40 / 100)

3. Measure the calculated volume of the 40% glycerol solution and add it to a container. Then, add distilled water to bring the final volume up to 100ml. Mix well to ensure uniformity.

b. 40ml of 0.5% agarose in 1X TBE solution:

1. Determine the amount of agarose needed:
- Desired final volume: 40ml
- Desired agarose concentration: 0.5%
- Formula weight of agarose: Approximatley 136.14g/mol

Use the same formula as before:
Amount (in grams) = (Desired concentration / 100) x Desired volume x Formula weight

Plugging in the values, we get:
Amount (in grams) = (0.5 / 100) x 40 x 136.14

2. Calculate the volume of the 10X TBE solution needed:
- Desired agarose concentration in 1X TBE solution: 0.5% (1X TBE is 10-fold diluted compared to 10X TBE)
- Concentration of 10X TBE solution: Unknown
- Volume of 10X TBE solution = Amount (in grams) / (Concentration / 100)

Plug in the values and solve for concentration:
Concentration = Amount (in grams) / (Volume of 1X TBE solution / 100)

Assuming you are using a 1X TBE solution, the concentration would be 10X. So, the volume of the 10X TBE solution needed would be 4ml (40ml / 10).

3. Measure the calculated volume of the 10X TBE solution and add it to a container. Then, add the calculated amount of agarose to the container. Make up the remaining volume with distilled water to a final volume of 40ml. Mix well to ensure proper dissolution.

Please note that these calculations provided are based on the given information and assumptions. It is always important to double-check your calculations and carry out proper laboratory techniques while preparing buffers.