A team of researchers do not use a full gene, but use a shortened version .. (which is coded for all necessary amino acids)

Can anyone describe how the shortened version of a gene may be obtained ??

or provide a source to help please

Thank you ....

Totally stuck !

To obtain a shortened version of a gene, researchers typically use a technique called gene truncation or gene fragmentation. This involves selectively removing or deleting specific sections or regions of the gene sequence to create a shorter version while still retaining the necessary information for coding the required amino acids.

Here is a step-by-step explanation of how the shortened version of a gene can be obtained:

1. Gene identification: The first step is to identify the target gene that needs to be shortened. Researchers usually have a specific gene in mind that is responsible for producing a particular protein of interest.

2. DNA extraction: Once the target gene is identified, DNA extraction is performed to isolate the genetic material containing the gene of interest. Various DNA extraction protocols are available and can be followed based on the source of the DNA (e.g., cells, tissues, blood, etc.).

3. Gene amplification: The isolated DNA containing the target gene is then amplified using a technique called polymerase chain reaction (PCR). PCR allows for the selective amplification of a specific DNA segment, greatly increasing its quantity for further analysis.

4. Designing primers: To truncate the gene, specific primers are designed. Primers are short DNA sequences that will bind to specific regions of the target gene. The primers should be designed in such a way that they flank the unnecessary sections that need to be removed, leaving behind the desired shortened version.

5. PCR amplification with primers: The amplified DNA from step 3 is then subjected to PCR amplification using the designed primers. The primers bind to their respective regions on the DNA template, and the polymerase enzyme extends the DNA strand, resulting in the replication and amplification of the target gene sequence.

6. Gel electrophoresis: After PCR amplification, gel electrophoresis can be done to visualize the amplified DNA fragments. This technique separates DNA fragments based on their size, allowing researchers to identify the specific fragment of interest.

7. Gene cloning: If necessary, the amplified shortened gene is cloned into an appropriate vector, such as a plasmid or a viral vector. This process involves inserting the gene fragment into the vector, which can then be amplified or expressed in host cells for further experiments.

It's worth noting that the specific techniques and protocols used for truncating a gene may vary depending on the research requirements and the preferences of the research group. If you need detailed, step-by-step instructions or specific protocols, it is recommended to consult scientific literature or research articles related to gene truncation.

Additionally, there are resources available online that provide detailed protocols and methodologies for gene truncation, such as molecular biology textbooks, scientific research journals, or websites that focus on molecular biology techniques like the National Center for Biotechnology Information (NCBI) or protocols.io. These sources can provide more specific information and guidance on obtaining a shortened version of a gene.