Tuesday
September 30, 2014

Homework Help: chemistry please help

Posted by stew on Saturday, February 16, 2008 at 2:31pm.

An enzyme sample contains 24 mg protein/mL. Of this sample, 20
microliters in a standard incubation volume of 0.1 mL catalyzed the
incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the
velocity of the reaction in terms of the following:

A)micromoles/minute
B)micromoles/liter/minute
C)micromoles/miligrams of protein/minute
D)Units/milliliter
E)Units/miligrams of protein


Of this sample, 50 mL were fractionated by ammonium sulfate
precipitation. The fraction precipitating between 30 and 50% saturation was
redissolved in a total volume of 10 mL and dialyzed. The solution after
dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction,
20 microliters catalyzed the reaction rate of 5.9 nmol/min under the
standard assay conditions. calculate the following:

A. The recovery of enzyme after the ammonium sulfate step.
B. The fold purification after the ammonium sulfate step.

note: for recovery: divide total units of he sample by the total units of the crude homogenate and multiply by 100.
for fold purification: it is the ratio of the specific activity of a given fraction divided by the specific activity of the crude homogenate.

Answer this Question

First Name:
School Subject:
Answer:

Related Questions

chemistry - An enzyme sample contains 24 mg protein/mL. Of this sample, 20 ...
biochemistry - An enzyme sample contains 24 mg protein/mL. Of this sample, 20 ...
chemistry - A 1.10g- sample contains only glucose and sucrose . When the sample ...
Biology - Hi, I am doing my lab assignment about Protein Analysis. Our job is to...
chemistry - A 1.10- sample contains only glucose and sucrose . When the sample ...
Chemistry - consider 2 sample of nitrogen gas. sample 1 contains 1.5 mol of N2 ...
Biochem - 1 ml of whole blood is collected and centrifuged to obtain a plasma ...
Biochem - 1 ml of whole blood is collected and centrifuged to obtain a plasma ...
chemistry - a sample of glucose, c6h12o6 contains 2.03*10^21 atoms of carbon. (a...
Chemistry - A sample of glucose, C6H12O6, contains 5.1x10623. a.)How many atoms ...

Search
Members