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April 16, 2014

Homework Help: chemistry

Posted by stew on Friday, February 15, 2008 at 7:29pm.

An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated by ammonium sulfate precipitation. The fraction precipitating between 30 and 50% saturation was redissolved in a total volume of 10 mL and dialyzed. The solution after dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction, 20 microliters catalyzed the reaction rate of 5.9 nmol/min under the standard assay conditions. calculate the following:

A. The recovery of enzyme after the ammonium sulfate step.
B. The fold purification after the ammonium sulfate step.

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