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May 24, 2015

Homework Help: chemistry

Posted by alan on Monday, February 4, 2008 at 8:54pm.

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the final pH?

If needed: TRIS(Trizma base) mw= 121.1; pka= 8.3;
TRIS-HCl mw= 157.6; pka= 8.3

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