Which one of the following is the proper/best way to utilize ...

";"
","
"and"

....

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; and Forensic DNA for Officers of the Court course; President’s DNA Initiative.

Or

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; Forensic DNA for Officers of the Court course; President’s DNA Initiative.

Or

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; and Forensic DNA for Officers of the Court course, President’s DNA Initiative.

Or

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course and Forensic DNA for Officers of the Court course; President’s DNA Initiative.

Or

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course and Forensic DNA for Officers of the Court course, President’s DNA Initiative.

I'm not clear on what you're asking. If you are listing three course names, here are my suggestions:

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course

What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course

Forensic DNA for Officers of the Court course: President’s DNA Initiative.

I apologize for the confusion. The three courses are:

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course

What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course

Forensic DNA for Officers of the Court course

And the place that provided these three courses:

President's DNA Initiative

If I were to combine the names of the curses together, would I have to put a ";" at the end of each course, for example:

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; Forensic DNA for Officers of the Court course

And then add "President's DNA Initiative" after a ",":

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; Forensic DNA for Officers of the Court course, President's DNA Initiative.

Other ways of combining the three courses along with "President's DNA Initiative" after that, considering where to put the ";" "," "and" exactly between each course are on the previous post.

I hope you understand.

Are you supposed to correct something that was given to you?

Or are you writing this yourself and want to put them in the correct form?

??

I am writing this myself and want to put them in the correct form. It is following the same idea that you mentioned in another post you answered.

What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course

What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course

Forensic DNA for Officers of the Court course

And the place that provided these three courses:

President's DNA Initiative

Then I would write them like this, in a bulletted list:

President's DNA Initiative courses:
~What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course
~What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course
~Forensic DNA for Officers of the Court course

AND ... delete the word "course" at the end of each of the three listed.

=)

The proper way to utilize punctuation in this case is by using the semicolon (;) to separate the different courses, the comma (,) to separate the different elements within each course, and the word "and" to indicate the last element within each course.

So, the correct way to write the given sentence is:

"What Every Law Enforcement Officer Should Know About DNA Evidence: First Responding Officers course; What Every Law Enforcement Officer Should Know About DNA Evidence: Investigators and Evidence Technicians course; and Forensic DNA for Officers of the Court course; President’s DNA Initiative."

This punctuation usage makes it clear that there are three separate courses: "First Responding Officers course," "Investigators and Evidence Technicians course," and "Forensic DNA for Officers of the Court course." The phrase "President’s DNA Initiative" is not a course; it is separate and additional information related to the previous information.

Using this punctuation structure ensures that the sentence is clear and avoids any confusion about the different elements or courses being described.