The following data describe the binding of oxygen to human myoglobin at 37oC.

P02 (mm Hg) = 0.5 1 2 3 4
Theta = .161 .277 .434 .535 .605

P02 (mm Hg) = 6 8 12 20
Theta = .697 .754 .821 .885

From these data, estimate:

a). P50
b) the fraction saturation of myoglobin at 30 mm, the partial pressure of O2 in venous blood.

I don’t know how to start and go about solving this problem.

Should I use this formula to calculate P50? Y=[S]^n / [S]^n + [S50]^n

Some please help. The following data describe the binding of oxygen to human myoglobin at 37oC.

P02 (mm Hg) = 0.5 1 2 3 4
Theta = .161 .277 .434 .535 .605

P02 (mm Hg) = 6 8 12 20
Theta = .697 .754 .821 .885

From these data, estimate:

a). P50
b) the fraction saturation of myoglobin at 30 mm, the partial pressure of O2 in venous blood.

I don’t know how to start and go about solving this problem.

Should I use this formula to calculate P50? Y=[S]^n / [S]^n + [S50]^n

Some please help.

Thank you for using the Jiskha Homework Help Forum. but instead of just labeling this HELP, please label it math, chem, or whatever the subject matter is. Most of us are looking for certain areas to read.

your numbers make no sense, what are they estimated to be?

To estimate P50 and the fraction saturation of myoglobin at 30 mm, we can use the Hill equation. The Hill equation is commonly used to describe the binding of oxygen to hemoglobin and myoglobin.

The Hill equation is: Y = [S]^n / ([S]^n + [S50]^n), where:
- Y is the fractional saturation of the protein.
- [S] is the concentration of the ligand (in this case, the partial pressure of O2, P02).
- [S50] is the ligand concentration at which the protein is 50% saturated.
- n is the Hill coefficient, which describes the cooperativity of binding.

To estimate P50, we need to find the P02 at which the fractional saturation is 50%. This corresponds to Y = 0.5 in the Hill equation.

Let's calculate P50 using the provided data:

First, plot the data points (P02 vs. Theta) on a graph. Use these data points to estimate the value of P02 at which Theta is 0.5 (or 50%). This estimated P02 will be our estimate for P50.

Second, calculate the fractional saturation (Theta) at 30 mm P02 using the Hill equation. Plug in the P02 value of 30 mm, the estimated P50, and the corresponding Theta value from the graph. Calculate the value of Y using the Hill equation.

Let's proceed with calculating P50 and the fractional saturation at 30 mm P02.