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December 22, 2014

Homework Help: molecular biology

Posted by mae on Wednesday, September 26, 2007 at 4:18pm.

How did O'Donnell show that the sliding clamp complex actually slides along dsDNA providing processivity to DNA Pol III?

I think he took dna, a primer, and one radioactive dNTPs, added Pol III that was isolated, ran a gel, and the conclusion was that it was processive because on the gel, the distributive section had many bands and processive had one band. I dunno. does anyone know what the experiment was called and what the hypothesis was? Please, I want serious answers

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