how would you make a 4% percent paraformaldehyde solution?
Work:
starting from 20% (diluted with PBS)
V1C1=V2C2
(100)(20)=(x)(4)
x=500
so add
496 ml of PBS (accounting for 4% paraformaldehyde) or would you add 500ml of PBS
Thank you
To make a 4% paraformaldehyde solution starting from a 20% solution (diluted with PBS), you can use the formula V1C1 = V2C2, where V1 represents the volume of the concentrated solution, C1 represents the concentration of the concentrated solution, V2 represents the volume of the diluted solution, and C2 represents the desired concentration of the diluted solution.
In this case, you have:
V1 = 100 ml (20% paraformaldehyde solution)
C1 = 20% (0.20)
C2 = 4% (0.04)
V2 represents the volume of the diluted solution you need to find. Rearranging the formula, you have:
V2 = (V1*C1) / C2
V2 = (100 ml * 0.20) / 0.04
V2 = 500 ml
Therefore, you would need to add 496 ml of PBS (accounting for the 4% paraformaldehyde) to reach a total volume of 500 ml. This is because the concentrated solution already contains 4 ml of paraformaldehyde, and when you add 496 ml of PBS, the final volume will be 500 ml with a 4% paraformaldehyde concentration.