Is the the rf value of lycopene and with what solvent can you use as a mobile phase on a TLC?

I don't know the answer here but I looked on the Internet and found something that may get you started. I found the Rf value for lycopene to be between 0.4 and 0.6 and the best mobile phase to be a mixture of polar/non-polar solvent. I couldn't find specifics.

the answer is c

the rf value depends on the kind of plate used (silica, alumina) and the mobile phase or developing solvent used. Lycopene has a very high Rf (~0.9) on silica gel when petroleum ether (75%) and methylene chloride (25%)is used as mobile phase.

The Rf value depends on the intra- and extra- cellular fluids which make up the cell. When you divide .75 by .25 you get the mole (m.)

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To determine the Rf (retention factor) value of lycopene, you can perform Thin Layer Chromatography (TLC). TLC involves separating and analyzing components of a mixture based on their different affinities for a stationary phase and a mobile phase.

To determine the Rf value, you will need to create a TLC plate. Begin by preparing a solution of lycopene and applying a small spot of the solution to a TLC plate. Next, choose a suitable solvent to act as the mobile phase.

In the case of lycopene, the best solvent system for the mobile phase is typically a mixture of polar and non-polar solvents. Commonly used mixtures include acetone/hexane, petroleum ether/acetone, or ethyl acetate/hexane.

To determine the specific solvent mixture, you can experiment with different combinations and ratios to achieve an optimal separation of lycopene. Start with equal volumes of the polar and non-polar solvents, and adjust the ratio as needed.

Once you have prepared the TLC plate and chosen the mobile phase solvent mixture, carefully place the plate in a developing chamber. Ensure that the solvent level is below the starting line on the plate, so your spot does not dissolve into the solvent.

Allow the solvent to travel up the TLC plate by capillary action, ensuring that the plate is kept in a closed chamber to avoid exposure to air and any external contaminants. Once the solvent front has nearly reached the top of the plate, remove the plate from the developing chamber and let it air dry.

Finally, visualize the separated spots on the TLC plate using either ultraviolet (UV) light or by using a visualization reagent specific for lycopene, such as iodine vapor or a dye staining solution. Measure the distance your lycopene spot traveled and calculate the Rf value using the formula: Rf = distance traveled by compound / distance traveled by solvent front.

It's important to note that the reported Rf value for lycopene can vary slightly depending on experimental conditions, such as the specific solvent mixture and TLC plate material. Thus, it is recommended to conduct multiple trials to obtain an average Rf value for lycopene.