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April 1, 2015

Homework Help: column chromatography

Posted by sue on Sunday, February 18, 2007 at 12:27pm.

A student loaded a mixture, dissolved in 1cm3 of dichloromethane, CH2Cl2, onto a small flash chromatography column and then proceeded to elute with hexanes/ethyl acetate 10:1. He/she found that all of the mixture came off in the first 4 fractions, with no separation. What technical mistake did the student make?

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